EFFECT OF l -CYSTINE ON INITIATION OF ANAEROBIC GROWTH OF ESCHERICHIA COLI AND AEROBACTER AEROGENES

Author:

Gorini Luigi1

Affiliation:

1. Department of Bacteriology and Immunology, Harvard Medical School, Boston 15, Massachusetts

Abstract

Gorini, Luigi (Harvard Medical School, Boston, Mass.). Effect of l -cystine on initiation of anaerobic growth of Escherichia coli and Aerobacter aerogenes . J. Bacteriol. 82: 305–312. 1961.—Under anaerobic conditions Escherichia coli and Aerobacter aerogenes , inoculated in a mineral-citrate-glucose medium at densities up to 10 6 bacteria per ml, exhibit a long lag, or fail to initiate growth at all. Growth is initiated rapidly if the medium is supplemented with various SH or SS compounds. Of these the most active is l -cystine, which is fully effective at 1 to 2 μ m . In a heavily seeded semisolid medium without cystine, turbidity rapidly appears at the aerobic surface and then slowly extends throughout the anaerobic region of the culture. This finding implies that a sufficiently dense anaerobically growing culture creates conditions in the medium which eliminate the requirement for cystine. The nature of this effect on the medium has not been determined, but certain possibilities (pH, pCO 2 ) have been eliminated. The anaerobic cystine requirement becomes more pronounced in the presence of Cu ++ , at concentrations far lower than those required for inhibition under aerobic conditions. While it is possible that cystine is acting by complexing the toxic metal ion, it seems more likely that l -cystine is an essential metabolite, poorly produced under anaerobic conditions, and that the marked toxicity of Cu ++ under anaerobic conditions depends on its complexing of cystine.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference4 articles.

1. Recent applications of genetic methods to the study of intermediary metabolism in microorganisms;DAVIS B. D.;Intern. Congr. Microbiol. 6th Congr. (Rome), p.,1953

2. Mutants of Escherichia coli requiring methionine or vitamin B12;DAVIS B. D.;J. Bacteriol.,1950

3. Necessite des orthodiphenols pour la croissance de Coccus P (Sarcina Sp.) Biochim. et Biophys;GORINI L.;Acta,1956

4. SH compounds in mitosis. II. The effect of mercaptoethanol on the structure of the mytotic apparatus in sea urchin eggs;MAZIA D.;Exptl. Cell Research,1958

Cited by 13 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3