Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology-Reference-Cited by-同舟云学术

Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology

Published:2016-11 Issue:11 Volume:54 Page:2701-2706
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hayden Randall T.¹,Clinton Lani K.²,Hewitt Carolyn¹,Koyamatsu Terri³,Sun Yilun⁴,Jamison Ginger¹,Perkins Rosalie¹,Tang Li⁴,Pounds Stanley⁴,Bankowski Matthew J.³⁵

Affiliation:

1. St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA

2. Pathology Residency Program, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA

3. Diagnostic Laboratory Services, Inc. (The Queen's Medical Center), Aiea, Hawaii, USA

4. St. Jude Children's Research Hospital, Department of Biostatisics, Memphis, Tennessee, USA

5. Department of Pathology and Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA

Abstract

ABSTRACT The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01475-16

Reference31 articles.

1. The Influence of Inadequate Antimicrobial Treatment of Bloodstream Infections on Patient Outcomes in the ICU Setting

2. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock*

3. Impact of an Antimicrobial Utilization Program on Antimicrobial Use at a Large Teaching Hospital A Randomized Controlled Trial

4. Point-prevalence study of inappropriate antibiotic use at a tertiary Australian hospital

5. Antimicrobial use in the ICU: Indications and accuracy-an observational trial

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in Escherichia coli;Antibiotics;2024-07-20

2. Relative quantification of the recA gene for antimicrobial susceptibility testing in response to ciprofloxacin for pathogens of concern;Scientific Reports;2024-02-01

3. Single-Cell Optical Nanomotion of Candida albicans in Microwells for Rapid Antifungal Susceptibility Testing;Fermentation;2023-04-07

4. Trends in the Determination of Antimicrobial Resistance in Aquaculture and Fisheries;Handbook on Antimicrobial Resistance;2023

5. Nano-Bio-Analytical Systems for the Detection of Emerging Infectious Diseases;Surface Engineering and Functional Nanomaterials for Point-of-Care Analytical Devices;2023