Identification of a Third Metalloprotease Toxin Gene in Extraintestinal Isolates of Bacteroides fragilis-Reference-Cited by-同舟云学术

Identification of a Third Metalloprotease Toxin Gene in Extraintestinal Isolates of Bacteroides fragilis

Published:1999-09 Issue:9 Volume:67 Page:4945-4949
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Chung Gyung-Tae¹,Franco Augusto A.²,Wu Shaoguang²,Rhie Gi-Eun¹,Cheng Rodney²,Oh Hee-Bok¹,Sears Cynthia L.²

Affiliation:

1. Laboratory of Bacterial Toxins, Department of Microbiology, National Institute of Health, Seoul, Korea,1 and

2. Divisions of Infectious Diseases and Gastroenterology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland2

Abstract

ABSTRACT To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene ( bft ) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.67.9.4945-4949.1999

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