Use of Tetrazolium Salts for Electron Transport Studies in Meningopneumonitis I. Reduced Nicotinamide Adenine Dinucleotide System

Author:

Allen Emma G.1

Affiliation:

1. Department of Microbiology and Immunology, State University of New York College of Medicine, Downstate Medical Center, Brooklyn, New York

Abstract

Allen, Emma G. (Downstate Medical Center, Brooklyn, N.Y.). Use of tetrazolium salts for electron transport studies in meningopneumonitis. I. Reduced nicotinamide adenine dinucleotide system. J. Bacteriol. 90: 1505–1512. 1965.—Purified preparations of meningopneumonitis virus (MP) prepared from allantoic fluids of infected chick embryo reduce several tetrazolium salts in the presence of reduced nicotinamide adenine dinucleotide under both aerobic and anaerobic conditions. The pattern of reduction by MP differs from that seen in normal allantoic membrane homogenates, and is inhibited by several cations but not by KCN, atabrine, amytal, antimycin A, or 2,3-dimercaptopropanol (BAL). The reduction of cytochrome c by purified preparations of MP differs from its reduction of tetrazolium salts in that the cytochrome reaction is completely inhibited by BAL and partially inhibited by amytal, atabrine, and antimycin A. The cytochrome reductase of normal allantoic membrane preparations is completely inhibited by each of these compounds.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference12 articles.

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2. Studies on the psittacosislymphogranuloma group. III. The effect of aureomycin;ALLEN E. G.;J. Exptl. Med.,1953

3. CIBA FOUNDATION. 1960. Quinones in electron transport. Little Brown & Co. Boston p. 130 346. J. BACTERIOL.

4. Tetrazolium salt as a seed germination indicator;COTTRELL H. J.;Nature,1947

5. Subcelluilar cultivation of a virus: growth of ornithosis virus in non-nucleate cytoplasm;CROCKER T.;Virology,1963

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