Use of Dialyzing Culture Technique for High Yield of Mycoplasma

Abstract

Pollock, Mary E. (University of Minnesota, Minneapolis). Use of dialyzing culture technique for high yield of Mycoplasma . J. Bacteriol. 90: 1682–1685. 1965.—The saprophytic pleuropneumonia-like organism, Mycoplasma laidlawii , type A, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. Generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. A defined mixture, which alone would not support growth of Mycoplasma , could replace the complex soy peptone-yeast extract medium in the inner membrane vessel of the dialyzing culture flask with no decrease in growth rate or yield.