Diversity Dynamics of Marine Bacteria Studied by Immunofluorescent Staining on Membrane Filters

Author:

Dahle Arne B.1,Laake Morten1

Affiliation:

1. Institute of Microbiology and Plant Physiology, University of Bergen, Bergen, Norway

Abstract

Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black Uni-Pore membrane filters by an indirect immunofluorescent staining procedure. The method proved to be very specific and practical for use in a large-scale field sampling program. Growth of all five isolates was stimulated by high values for net primary production, chlorophyll a , and dissolved organic carbon. Calculation of a diversity index based on specific and total counts is proposed as a way of characterizing the dynamics of organotrophic bacterial populations in the sea.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference51 articles.

1. Secretion of vitamins and amino acids into the environment by Ochromonas danica;Aaronsen S.;J. Phycol.,1971

2. Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining;Apel W. A.;Appl. Environ. Microbiol.,1976

3. Bain N. and J. M. Shewan. 1968. Identification of Aeromonas Vibrio and related organisms p. 79-84. In B. M. Gibbs and D. A. Shapton (ed.) Identification methods for microbiologists part B. Academic Press Inc. New York.

4. Selective stimulation of marine bacteria by algal extracellular products;Bell W. H.;Limnol. Oceanogr.,1974

5. Chemotactic and growth responses of marine bacteria to algal extracellular products;Bell W. H.;Biol. Bull.,1972

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3