Testing of Diagnostic Methods for Detection of Influenza Virus for Optimal Performance in the Context of an Influenza Surveillance Network-Reference-Cited by-同舟云学术

Testing of Diagnostic Methods for Detection of Influenza Virus for Optimal Performance in the Context of an Influenza Surveillance Network

Published:2007-09 Issue:9 Volume:45 Page:3109-3110
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pérez-Ruiz Mercedes¹,Yeste Ruth¹,Ruiz-Pérez María-José¹,Ruiz-Bravo Alfonso²,de la Rosa-Fraile Manuel¹,Navarro-Marí José María¹

Affiliation:

1. Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain

2. Departamento de Microbiología, Universidad de Granada, Granada, Spain

Abstract

ABSTRACT Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strain.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00697-07

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