Complete Genome Sequence of Rhodococcus sp. Strain W8901, a Human Clinical Specimen, Assembled Using MiSeq and MinION Sequence Data

Author:

Gulvik Christopher A.1,Batra Dhwani2,Howard Dakota T.2,Sheth Milli2,Humrighouse Ben W.1,Lee Justin2,McQuiston John R.1,Lasker Brent A.1ORCID

Affiliation:

1. Bacterial Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Abstract

Rhodococcus sp. strain W8901 is a Gram-positive, aerobic, mycolic acid-containing coccobacillus obtained from a patient with acute lymphocytic leukemia. Here, we report on the complete, circular genome sequence obtained using Illumina MiSeq and Oxford Nanopore Technologies MinION reads in order to better resolve the phylogeny of a rare pathogen.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference19 articles.

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3. Current taxonomy of Rhodococcus species and their role in infections

4. Next-generation systematics: An innovative approach to resolve the structure of complex prokaryotic taxa

5. Investigation of an apparent outbreak of Rhodococcus equi bacteremia

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