Affiliation:
1. Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia
Abstract
ABSTRACT
An improved transposon (ThD
73
) for
Haloarcula hispanica
is described. Based on the halobacterial insertion sequence ISH
28
, it showed little target sequence specificity but was biased toward a lower G+C content. Twenty randomly selected ThD
73
mutants were analyzed, and the DNA flanking their insertions revealed several recognizable sequences, including two (unrelated) ISH elements.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference19 articles.
1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
2. Berg
C. M.
Berg
D. E.
Uses of transposable elements and maps of known insertions
Escherichia coli and Salmonella typhimurium: cellular and molecular biology.
Neidhardt
F. C.
Ingraham
J. L.
Low
K. B.
Magasanik
B.
Schaechter
M.
Umbarger
H. E.
1987
1071
1109
American Society for Microbiology
Washington D.C.
3. Berg
C. M.
Berg
D. E.
Groisman
E. A.
Transposable elements and the genetic engineering of bacteria
Mobile DNA.
Berg
D. E.
Howe
M. M.
1989
879
925
American Society for Microbiology
Washington D.C.
4. Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions
5. Transformation methods for halophilic archaebacteria;Cline S. W.;Can. J. Microbiol.,1989
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献