Author:
Felton J,Michaelis S,Wright A
Abstract
Escherichia coli K-12 has two genes, asnA+ and asnB+, either one of which is able to satisfy the need of cells for asparagine. In order for a strain to have an auxotrophic requirement for asparagine, both genes must be mutationally inactivated. We obtained mutants with Tn5 inserted in asnB. asnB was mapped by conjugation and by three-factor P1 transductions at 15 min on the E coli K-12 linkage map, between ubiF and nagB. Specialized transducing phage lamba 781 supE was shown to carry asnB, as well as supE, ubiF, nagA, and nagB. asnA is the previously mapped ilv-linked asn locus, whiich is between uncB and rbs. E. coli C also has two asn genes, corresponding to asnA and asnB.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference36 articles.
1. Recalibrated linkage map of Escherichia coli K-12;Bachmann B. J.;Bacteriol. Rev.,1976
2. Berg D. E. 1977. Insertion and excision of the transposable kanamycin resistance determinant Tn 5 p. 205-212. In A. I. Bukhari J. A. Shapiro and S. L. Adhya (ed.) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y. 227
3. The construction in vitro of transducing derivatives of phage lambda;Borck K.;Mol. Gen. Genet.,1976
4. Production of Lasparaginase II by Escherichia coli;Cedar H.;J. Bacteriol.,1968
5. The asparagine synthetase of Escherichia coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products;Cedar H.;J. Biol. Chem.,1969