Affiliation:
1. Abteilung für Mikrobiologie und Molekularbiologie der Universität Hohenheim, Stuttgart-Hohenheim, Germany
Abstract
Supercoiled minicircular deoxyribonucleic acid (DNA) molecules with molecular weights of 1.8 × 10
6
and 2.3 × 10
6
have been isolated from two wild strains of
Escherichia coli
. DNA-DNA hybridization experiments indicate that these DNA molecules share extended homologies with the minicircular DNA of
E. coli
15. The DNA of the colicinogenic factor E1 (ColE1) also hybridizes to a large extent with minicircular DNA of
E. coli
15. In contrast, no hybridization could be detected with various large extrachromosomal DNA elements such as the colicinogenic factor V (ColV), the beta-hemolytic factor (Hly), or the P1-like DNA of
E. coli
15. Two different insertion DNA species of
E. coli
integrated into λdg-DNA (λdg UP
in
128, λdg UP
in
308) do not show any annealing with minicircular DNA of
E. coli
15.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
23 articles.
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