Mutation of Influenza A Virus PA-X Decreases Pathogenicity in Chicken Embryos and Can Increase the Yield of Reassortant Candidate Vaccine Viruses

Author:

Hussain Saira1,Turnbull Matthew L.1,Wise Helen M.1,Jagger Brett W.23,Beard Philippa M.14,Kovacikova Kristina1,Taubenberger Jeffery K.3,Vervelde Lonneke1,Engelhardt Othmar G.5,Digard Paul1ORCID

Affiliation:

1. The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom

2. Department of Pathology, University of Cambridge, Cambridge, United Kingdom

3. National Institutes of Health, Bethesda, Maryland, USA

4. The Pirbright Institute, Pirbright, Surrey, United Kingdom

5. National Institute for Biological Standards and Control, South Mimms, Hertfordshire, United Kingdom

Abstract

Influenza A virus is a widespread pathogen that affects both humans and a variety of animal species, causing regular epidemics and sporadic pandemics, with major public health and economic consequences. A better understanding of virus biology is therefore important. The primary control measure is vaccination, which for humans mostly relies on antigens produced in eggs from PR8-based viruses bearing the glycoprotein genes of interest. However, not all reassortants replicate well enough to supply sufficient virus antigen for demand. The significance of our research lies in identifying that mutation of the PA-X gene in the PR8 strain of virus can improve antigen yield, potentially by decreasing the pathogenicity of the virus in embryonated eggs.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Department of Health

RCUK | Biotechnology and Biological Sciences Research Council

HHS | Biomedical Advanced Research and Development Authority

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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