Light Response of Pseudomonas putida KT2440 Mediated by Class II LitR, a Photosensor Homolog

Author:

Sumi Satoru1,Mutaguchi Naotaka1,Ebuchi Teppei1,Tsuchida Hiroaki1,Yamamoto Takahiro1,Suzuki Maki1,Natsuka Chihiro1,Shiratori-Takano Hatsumi1,Shintani Masaki2ORCID,Nojiri Hideaki34ORCID,Ueda Kenji1,Takano Hideaki1ORCID

Affiliation:

1. Life Science Research Center, College of Bioresource Sciences, Nihon University, Tokyo, Japan

2. Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, Hamamatsu, Shizuoka, Japan

3. Biotechnology Research Center, The University of Tokyo, Tokyo, Japan

4. Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan

Abstract

The LitR/CarH family is a new group of photosensor homologous to MerR-type transcriptional regulators. Proteins of this family are distributed to various nonphototrophic bacteria and grouped into at least five classes (I to V). Pseudomonas putida retaining three class II LitR proteins exhibited a genome-wide response to light. All three paralogs were functional and mediated photodependent activation of promoters directing the transcription of light-induced genes or operons. Two LOV (light, oxygen, or voltage) domain proteins, adjacently encoded by two litR genes, were also essential for the photodependent transcriptional control. Despite the difference in light-sensing mechanisms, the DNA binding consensus of class II LitR [T(G/A)TA(C/T)A] was the same as that of class I. This is the first study showing the actual involvement of class II LitR in light-induced transcription.

Funder

Grant-in-Aid for Scientific Research

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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