Affiliation:
1. Institute of Medical Microbiology and Immunology, The Bartholin Building, Aarhus University, DK-8000 Aarhus C, Denmark
2. Mycoplasma Laboratory, Statens Serum Institut, DK-2300 Copenhagen S, Denmark
3. Department of Medical Protein Research and Biochemistry, Flanders Interuniversity Institute for Biotechnology and Ghent University, Ghent, Belgium
Abstract
ABSTRACT
Mycoplasma genitalium
causes nonchlamydial nongonococcal urethritis.
M. genitalium
was detected by PCR in 17 urethral swabs obtained from 99 men with and without urethritis (J. S. Jensen, R. Orsum, B. Dohn, S. Uldum, A. M. Worm, and K. Lind, Genitourin. Med. 69:265-269, 1993), and later, four
M. genitalium
strains were isolated (J. S. Jensen, H. T. Hansen, and K. Lind, J. Clin. Microbiol. 34:286-291, 1996). The objective of this study was to characterize immunogenic proteins of
M. genitalium
by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting by using a hyperimmune rabbit serum against
M. genitalium
G37, determine their identity by mass spectrometry, and develop an
M. genitalium
-specific enzyme-linked immunosorbent assay (ELISA) free from cross-reactivity with
M. pneumoniae
antibodies. Using recombinant fragments of the C-terminal part of MgPa (rMgPa), we developed a specific ELISA for detection of
M. genitalium
antibodies. This antigen did not bind
M. pneumoniae
antibodies. Using serum samples from the 99 men with and without urethritis, we found that 26 had immunoglobulin G (IgG) antibodies to
M. genitalium
. There was a strong statistically significant correlation between PCR and IgG antibodies to
M. genitalium
(odds ratio [OR], 5.9; 95% confidence interval [CI], 2.3 to 21.5;
P
= 0.002). Furthermore, men with recurrent urethritis were more likely to have antibodies to
M. genitalium
than were those without recurrent urethritis (OR, 4.0; 95% CI, 1.1 to 14.5;
P
= 0.0383) and they had significantly higher antibody titers. By use of the rMgPa ELISA, this study further substantiates the importance of
M. genitalium
as a cause of male urethritis.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
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