Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52

Author:

Tan Y1,Miller K J1

Affiliation:

1. Department of Food Science, Pennsylvania State University, University Park 16802.

Abstract

In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference27 articles.

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3. Rhizomucor miehei triglyceride lipase is synthesized as a precursor;Boel E.;Lipids,1988

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5. Overexpression of a thermostable lipase gene from Pseudomonas fluorescens in Eschenchia coli;Chung G. H.;Appl. Microbiol. Biotechnol.,1991

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