Immunochromatographic IgG/IgM Test for Rapid Diagnosis of Active Tuberculosis

Author:

Ben-Selma Walid1,Harizi Hedi1,Boukadida Jalel1

Affiliation:

1. Microbiology and Immunology Laboratory, UR02SP13, CHU Farhat Hached, Sousse, Tunisia

Abstract

ABSTRACT For rapid diagnosis and discrimination between active tuberculosis (TB) and other pulmonary diseases, we evaluated the clinical usefulness of detection of serum immunoglobulin IgG and IgM antibodies raised against mycobacterial 38-kDa, 16-kDa, and 6-kDa antigens by a commercial rapid immunochromatographic IgG/IgM test (Standard Diagnostics, South Korea) in 246 serum samples from three groups of patients: (i) 171 patients with active TB (128 with pulmonary TB [pTB] and 43 with extrapulmonary TB [epTB]), (ii) 73 patients with pulmonary non-TB diseases, and (iii) two leprosy patients. The sensitivities of IgG and IgM in patients with active TB (pTB and epTB) were 68.4% and 2.3%, respectively. IgG had the best performance characteristics, with sensitivities of 78.1% and 39.5% in sera from patients with active pTB and epTB, respectively, and a specificity of 100%. The sensitivities of IgM were poor and were similar for pTB and epTB (2.3%). In contrast, specificity was very elevated (100%). The combination of IgG with IgM did not improve its sensitivity. IgG-mediated responses against the mycobacterial 38-kDa, 16-kDa, and 6-kDa antigens might constitute a clinically useful tool for presumptive diagnosis and discrimination of active pTB from other pulmonary diseases. Moreover, based on its simplicity and rapidity of application, it could be a screening tool for active pTB in poorly equipped laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference47 articles.

1. Accuracy and utility of commercially available amplification and serologic tests for the diagnosis of minimal pulmonary tuberculosis;Al-Zahrani K.;Am. J. Respir. Crit. Care Med.,2000

2. Diagnostic standards and classification of tuberculosis in adults and children;American Thoracic Society;Am. J. Respir. Crit. Care Med.,2000

3. Sensitivity and specificity of a multi-antigen ELISA test for the serological diagnosis of tuberculosis;Amicosante M.;Int. J. Tuberc. Lung Dis.,1999

4. Uncommon presentation of tuberculosis: the potential value of a novel diagnostic assay based on the Mycobacterium tuberculosis-specific antigens ESAT-6 and CPF-10;Arend S. M.;Int. J. Tuberc. Lung Dis.,2001

5. Evaluation of the diagnostic value of measuring IgG, IgM, and IgA antibodies to mycobacterial A60 antigen in active tuberculosis;Ben-Selma W.;Diagn. Microbiol. Infect. Dis.,2010

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