Affiliation:
1. Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas
Abstract
ABSTRACT
The sputum (mucus) layer of the cystic fibrosis (CF) lung is a complex substrate that provides
Pseudomonas aeruginosa
with carbon and energy to support high-density growth during chronic colonization. Unfortunately, the CF lung sputum layer has been difficult to mimic in animal models of CF disease, and mechanistic studies of
P. aeruginosa
physiology during growth in CF sputum are hampered by its complexity. In this study, we performed chromatographic and enzymatic analyses of CF sputum to develop a defined,
s
ynthetic
CF
sputum
m
edium (SCFM) that mimics the nutritional composition of CF sputum. Importantly,
P. aeruginosa
displays similar phenotypes during growth in CF sputum and in SCFM, including similar growth rates, gene expression profiles, carbon substrate preferences, and cell-cell signaling profiles. Using SCFM, we provide evidence that aromatic amino acids serve as nutritional cues that influence cell-cell signaling and antimicrobial activity of
P. aeruginosa
during growth in CF sputum.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
499 articles.
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