Affiliation:
1. Biology Department, The Catholic University of America, Washington, D.C.
Abstract
Banville, Robert
R. (The Catholic University of America, Washington, D.C.). Factors affecting growth of
Staphylococcus aureus
L forms on semidefined medium. J. Bacteriol.
87:
1192–1197. 1964.—A semidefined agar medium was found suitable for production and cultivation of the L form of
Staphylococcus aureus
. In semidefined liquid medium, growth of the L form took place in the form of a sediment containing large masses of cells, but heavy and diffuse growth occurred in the same medium with 0.05% agar. The optimal pH for L-colony formation on solid medium was 6.5. More L colonies developed on 0.75% agar than at higher agar concentrations. L colonies developed in greater numbers on pour plates than on streak plates, and in some cases more L colonies appeared under anaerobic incubation. L-colony formation appeared to be inhibited by sodium citrate. The vitamin requirements of the L forms studied were similar to those of the classical form.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
14 articles.
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