Development of a Single-Cycle Infectious SARS-CoV-2 Virus Replicon Particle System for Use in Biosafety Level 2 Laboratories

Author:

Malicoat Johnny1,Manivasagam Senthamizharasi1,Zuñiga Sonia2,Sola Isabel2,McCabe Dianne1,Rong Lijun3,Perlman Stanley1ORCID,Enjuanes Luis2ORCID,Manicassamy Balaji1ORCID

Affiliation:

1. Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA

2. Coronavirus Laboratory, Departamento Biologia Molecular y Celular, Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain

3. Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA

Abstract

Due to the highly contagious nature of SARS-CoV-2 and the lack of immunity in the human population, research on SARS-CoV-2 has been restricted to biosafety level 3 laboratories. This has greatly limited participation of the broader scientific community in SARS-CoV-2 research and thus has hindered the development of vaccines and antiviral drugs.

Funder

COVID Pilot Grant - Carver Trust- University of Iowa

Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases

HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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2. Adapting High-Throughput Screening Methods and Assays for Biocontainment Laboratories

3. Federal Select Agent Program. Select agents and toxins exclusions: excluded attenuated strains of HHS select agents section 73.3 (e). https://www.selectagents.gov/sat/exclusions/hhs.htm.

4. Identification of interferon-stimulated genes that attenuate Ebola virus infection

5. Biosafety recommendations for work with influenza viruses containing a hemagglutinin from the A/goose/Guangdong/1/96 lineage;Gangadharan D;MMWR Recommend Rep,2013

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