Fatty Acid Synthesis Knockdown Promotes Biofilm Wrinkling and Inhibits Sporulation in Bacillus subtilis

Author:

Arjes Heidi A.1,Gui Haiwen1,Porter Rachel2,Atolia Esha1,Peters Jason M.3456ORCID,Gross Carol78,Kearns Daniel B.9ORCID,Huang Kerwyn Casey11011ORCID

Affiliation:

1. Department of Bioengineering, Stanford University School of Medicine, Stanford, California, USA

2. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, USA

3. Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin—Madison, Madison, Wisconsin, USA

4. Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin—Madison, Madison, Wisconsin, USA

5. Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin, USA

6. Department of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, Wisconsin, USA

7. Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, USA

8. Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, California, USA

9. Department of Biology, Indiana University, Bloomington, Indiana, USA

10. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA

11. Chan Zuckerberg Biohub, San Francisco, California, USA

Abstract

For many bacteria, life typically involves growth in dense, three-dimensional communities called biofilms that contain cells with differentiated roles held together by extracellular matrix. To examine how essential gene function varies between vegetative growth and the developmental states of biofilm formation and sporulation, we created and screened a comprehensive library of strains using CRISPRi to knockdown expression of each essential gene in the biofilm-capable Bacillus subtilis strain 3610.

Funder

HHS | National Institutes of Health

National Science Foundation

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3