Affiliation:
1. Departamento de Química Biológica, Facultad Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Abstract
ABSTRACT
We describe a new enzymatic functionality for the surface layer (S-layer) of
Lactobacillus acidophilus
ATCC 4356, namely, an endopeptidase activity against the cell wall of
Salmonella enterica
serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in
Bacillus subtilis
resulted in the functional verification of the enzymatic activity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
43 articles.
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