Affiliation:
1. Division of Life Sciences, King's College London, United Kingdom.
Abstract
Apparent oxygen affinities for Escherichia coli cells and membranes containing a terminal oxidase with only one type of ligand-binding heme, cytochrome o', were measured with oxyleghemoglobin and oxymyoglobin as sensitive oxygen reporters. Two Km values (0.15 to 0.35 microM and 0.016 to 0.085 microM) were detected, well below values determined for the purified oxidase by insensitive electrode methods.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference32 articles.
1. The respiratory system of Azotobacter vinelandii. 1. Properties of phosphorylating membranes;Ackrell B. A. C.;Eur. J. Biochem.,1971
2. Appleby C. A. and F. J. Bergersen. 1980. Preparation and experimental use of leghaemoglobin p. 315-335. In F. J. Bergersen (ed.) Methods of evaluating biological nitrogen fixation. John Wiley and Sons Ltd. Chichester United Kingdom.
3. Role of the transcriptional activator AppY in regulation of the cyx appA operon of Escherichia coli by anaerobiosis, phosphate starvation, and growth phase;Atlung T.;J. Bacteriol.,1994
4. Systems utilizing oxygenated leghaemoglobin and myoglobin as sources of free dissolved oxygen at low concentrations for experiments with bacteria;Bergersen F. J.;Anal. Biochem.,1979
5. Properties of terminal oxidase systems of bacteroids from root nodules of soybean and cowpea and of N2-fixing bacteria grown in continuous culture;Bergersen F. J.;J. Gen. Microbiol.,1980
Cited by
63 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献