Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria-Reference-Cited by-同舟云学术

Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria

Published:2008-07 Issue:13 Volume:74 Page:4231-4235
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gloess Stefanie¹,Grossart Hans-Peter¹,Allgaier Martin¹,Ratering Stefan²,Hupfer Michael¹

Affiliation:

1. Leibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany

2. Institute of Applied Microbiology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany

Abstract

ABSTRACT Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02545-07

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