Affiliation:
1. National Antimicrobial Resistance Surveillance Program at Women’s and Children’s Hospital1 and
2. Molecular Microbiology Unit, Women’s and Children’s Hospital,2 North Adelaide, South Australia 5006, Australia
Abstract
ABSTRACT
Enterococci with resistance to glycopeptides have recently emerged in Australia. We developed multiplex PCR assays for
vanA
,
vanB
,
vanC1
, and
vanC2
or
vanC3
in order to examine the genetic basis for vancomycin resistance in Australian isolates of vancomycin-resistant
Enterococcus faecium
and
E. faecalis
(VRE). The predominant genotype from human clinical
E. faecium
isolates was
vanB
. The PCR
van
genotype was consistent with the resistance phenotype in all but six cases. One
vanA E. faecalis
isolate had a VanB phenotype, one
vanB E. faecium
isolate had a VanA phenotype, and four
E. faecalis
isolates were consistently negative for
vanA
,
vanB
,
vanC1
, and
vanC2
or
vanC3
, even though they exhibited a VanB phenotype. These four isolates were subsequently examined for the presence of
vanD
by published methods and were found to be negative. No vancomycin-susceptible strains produced a PCR product. On the basis of our findings the epidemiology of VRE in Australia appears to be different from that in either the United States or Europe. Our multiplex PCR assays gave a rapid and accurate method for determining the genotype and confirming the identification of glycopeptide-resistant enterococci. Rapid and accurate methods are essential, because laboratory-based surveillance is critical in programs for the detection, control, and prevention of the transmission of glycopeptide-resistant enterococci.
Publisher
American Society for Microbiology
Reference23 articles.
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Etest technical guide 3B.
1995
AB Biodisk
Solna Sweden
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