Affiliation:
1. Biology Department, Concordia University, Montréal, Québec H3G 1M8, Canada
Abstract
ABSTRACT
Strains of
Escherichia coli
which lack the
dam
-encoded adenine methylase are mutators due to a reduction in the efficiency of postreplication mismatch repair. In this study, we show that Dam
−
strains are also defective in very-short-patch repair, the system which corrects T/G mismatches arising from the deamination of 5-methylcytosine. This defect is associated with decreased levels of Vsr, the endonuclease which initiates short-patch repair. We also show that production of the
dcm
-encoded cytosine methylase is unaffected in Dam
−
strains. Since the
dcm
and
vsr
genes are cotranscribed, the regulation of Vsr by Dam is probably posttranscriptional.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
27 articles.
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