Specificity and Topology of the Escherichia coli Xanthosine Permease, a Representative of the NHS Subfamily of the Major Facilitator Superfamily-Reference-Cited by-同舟云学术

Specificity and Topology of the Escherichia coli Xanthosine Permease, a Representative of the NHS Subfamily of the Major Facilitator Superfamily

Published:2001-08-15 Issue:16 Volume:183 Page:4900-4904
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Nørholm Morten H. H.¹,Dandanell Gert¹

Affiliation:

1. Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, 1307 Copenhagen K, Denmark

Abstract

ABSTRACT The specificity of XapB permease was compared with that of the known nucleoside transporters NupG and NupC. XapB-mediated xanthosine uptake is abolished by 2,4-dinitrophenol and exhibits saturation kinetics with an apparent K m of 136 μM. A 12-transmembrane-segment model was confirmed by translational fusions to alkaline phosphatase and the α fragment of β-galactosidase.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.16.4900-4904.2001

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