Microbial Origin of Plant-Type 2-Keto-3-Deoxy- d - arabino -Heptulosonate 7-Phosphate Synthases, Exemplified by the Chorismate- and Tryptophan-Regulated Enzyme from Xanthomonas campestris

Abstract

ABSTRACT Enzymes performing the initial reaction of aromatic amino acid biosynthesis, 2-keto-3-deoxy- d - arabino -heptulosonate 7-phosphate (DAHP) synthases, exist as two distinct homology classes. The three classic Escherichia coli paralogs are AroA I proteins, but many members of the Bacteria possess the AroA II class of enzyme, sometimes in combination with AroA I proteins. AroA II DAHP synthases until now have been shown to be specifically dedicated to secondary metabolism (e.g., formation of ansamycin antibiotics or phenazine pigment). In contrast, here we show that the Xanthomonas campestris AroA II protein functions as the sole DAHP synthase supporting aromatic amino acid biosynthesis. X. campestris AroA II was cloned in E. coli by functional complementation, and genes corresponding to two possible translation starts were expressed. We developed a 1-day partial purification method (>99%) for the unstable protein. The recombinant AroA II protein was found to be subject to an allosteric pattern of sequential feedback inhibition in which chorismate is the prime allosteric effector. l -Tryptophan was found to be a minor feedback inhibitor. An N-terminal region of 111 amino acids may be located in the periplasm since a probable inner membrane-spanning region is predicted. Unlike chloroplast-localized AroA II of higher plants, X. campestris AroA II was not hysteretically activated by dithiols. Compared to plant AroA II proteins, differences in divalent metal activation were also observed. Phylogenetic tree analysis shows that AroA II originated within the Bacteria domain, and it seems probable that higher-plant plastids acquired AroA II from a gram-negative bacterium via endosymbiosis. The X. campestris AroA II protein is suggested to exemplify a case of analog displacement whereby an ancestral aroA I species was discarded, with the aroA II replacement providing an alternative pattern of allosteric control. Three subgroups of AroA II proteins can be recognized: a large, central group containing the plant enzymes and that from X. campestris , one defined by a three-residue deletion near the conserved KPRS motif, and one possessing a larger deletion further downstream.