One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies-Reference-Cited by-同舟云学术

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

Published:2012-08 Issue:15 Volume:78 Page:5440-5443
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Jeong Jae-Yeon¹,Yim Hyung-Soon¹,Ryu Ji-Young¹,Lee Hyun Sook¹,Lee Jung-Hyun¹,Seen Dong-Seung²,Kang Sung Gyun¹

Affiliation:

1. Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, Ansan, South Korea

2. Macrogen Inc., World Meridian Venture Center, Geumchun-Gu, Seoul, South Korea

Abstract

ABSTRACT We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00844-12

Reference15 articles.

1. Ligation-independent cloning of PCR products (LIC-PCR)

2. Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones;Cheo DL;Genome Res.,2004

3. Enzyme free cloning for high throughput gene cloning and expression;de Jong RN;J. Struct. Funct. Genomics,2006

4. Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase

5. Genetic techniques for Thermococcus kodakarensis;Hileman TH;Front. Microbiol.,

Cited by 344 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Repurposing myoglobin into a carbene transferase for a [2,3]-sigmatropic Sommelet-Hauser rearrangement;Journal of Inorganic Biochemistry;2024-11

2. The actin-spectrin submembrane scaffold restricts endocytosis along proximal axons;Science;2024-08-23

3. Human FASTK preferentially binds single-stranded and G-rich RNA;2024-07-17

4. Mushroom Tyrosinase: Six Isoenzymes Catalyzing Distinct Reactions;ChemBioChem;2024-07-03

5. TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice;Nature Communications;2024-06-22