Advantage of Using Inosine at the 3′ Termini of 16S rRNA Gene Universal Primers for the Study of Microbial Diversity
Author:
Affiliation:
1. Department of Biotechnology Engineering
2. National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel
Abstract
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Link
https://journals.asm.org/doi/pdf/10.1128/AEM.00849-06
Reference30 articles.
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2. Batzer, M. A., J. E. Carlton, and P. L. Deininger. 1991. Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus. Nucleic Acids Res.19:5081.
3. Crick, F. H. C. 1966. Codon-anticodon pairing: the wobble hypothesis. J. Mol. Biol.19:548-555.
4. Curtis, T. P., W. T. Sloan, and J. W. Scannell. 2002. Estimating prokaryotic diversity and its limits. Proc. Natl. Acad. Sci. USA99:10494-10499.
5. Daly, K., R. J. Sharp, and A. J. McCarthy. 2000. Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria. Microbiology146:1693-1705.
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