Multicenter Evaluation of a Modified Protocol for the RAPIDEC Staph System for Direct Identification of Staphylococcus aureus in Blood Cultures-Reference-Cited by-同舟云学术

Multicenter Evaluation of a Modified Protocol for the RAPIDEC Staph System for Direct Identification of Staphylococcus aureus in Blood Cultures

Published:1998-12 Issue:12 Volume:36 Page:3707-3709
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

van Griethuysen Arjanne¹,Buiting Anton¹,Goessens Wil²,van Keulen Peter³,Wintermans Rob⁴,Kluytmans Jan³

Affiliation:

1. Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg,1

2. Department of Clinical Microbiology University Hospital Dijkzigt, Rotterdam,2 and

3. Department of Clinical Microbiology St. Ignatius Hospital and Hospital de Baronie, Breda,3

4. Department of Clinical Microbiology St. Franciscus Hospital, Roosendaal, and Hospital Lievensberg, Bergen op Zoom,4 The Netherlands

Abstract

ABSTRACT A modified protocol for the RAPIDEC Staph system (bioMérieux, Marcy-l’Etoile, France) for direct identification of Staphylococcus aureus in blood cultures was evaluated in a multicenter study. A total of 129 blood cultures (BACTEC 9000 Blood Culture System; Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) containing gram-positive cocci in clusters were analyzed by conventional methods and by RAPIDEC Staph in accordance with the manufacturer’s protocol and in accordance with a modified protocol. The sensitivity, specificity, and positive and negative predictive values obtained with the manufacturer’s protocol were 90.5, 97.7, 95.0, and 95.5%, respectively, and those obtained with the modified protocol were 100, 96.6, 93.3, and 100%, respectively. The modified protocol for the RAPIDEC Staph is easier to perform than the manufacturer’s protocol and is very reliable.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.12.3707-3709.1998

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