Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue-Reference-Cited by-同舟云学术

Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue

Published:1998-11 Issue:11 Volume:36 Page:3423-3425
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Satoh Yukio¹,Takasaka Noriko¹,Hoshikawa Yoshiko¹,Osaki Mitsuhiko²,Ohfuji Satoshi²,Ito Hisao²,Kaibara Nobuaki³,Kurata Takeshi⁴,Sairenji Takeshi¹⁴

Affiliation:

1. Departments of Biosignaling,1

2. Pathology,2 and

3. Surgery,3 Faculty of Medicine, Tottori University, Yonago 683, and

4. Department of Pathology, National Institute of Infectious Diseases, Tokyo 162,4 Japan

Abstract

ABSTRACT An association between Epstein-Barr virus (EBV) and gastric carcinoma has been studied through the EBV genome present in the carcinoma cells. Recently, we found that EBV DNA in paraffin-embedded gastric carcinoma tissue was detected effectively by PCR after pretreatment of the extracted DNA with a restriction enzyme, Bam HI or Eco RI. Here, we show that the PCR amplification was also enhanced by pretreatment of the DNA with other restriction enzymes or with bovine serum albumin and several other proteins. Treatment with these proteins may remove a PCR inhibitor(s) in the DNA samples extracted from the paraffin blocks.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.11.3423-3425.1998

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