Identification of Streptococci to Species Level by Sequencing the Gene Encoding the Manganese-Dependent Superoxide Dismutase

Author:

Poyart Claire1,Quesne Gilles1,Coulon Stephane1,Berche Patrick1,Trieu-Cuot Patrick1

Affiliation:

1. Laboratoire Mixte Pasteur-Necker de Recherche sur les Streptocoques et Streptococcies, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France

Abstract

ABSTRACT We have used a PCR assay based on the use of degenerate primers in order to characterize an internal fragment ( sodA int ) representing approximately 85% of the genes encoding the manganese-dependent superoxide dismutase in various streptococcal type strains ( S. acidominimus , S. agalactiae , S. alactolyticus , S. anginosus , S. bovis , S. constellatus , S. canis , S. cricetus , S. downei , S. dysgalactiae , S. equi subsp. equi , S. equi subsp. zooepidemicus , S. equinus , S. gordonii , S. iniae , S. intermedius , S. mitis , S. mutans , S. oralis , S. parasanguis , S. pneumoniae , S. porcinus , S. pyogenes , S. salivarius , S. sanguis , S. sobrinus , S. suis , S. thermophilus , and S. vestibularis ). Phylogenetic analysis of these sodA int fragments yields an evolutionary tree having a topology similar to that of the tree constructed with the 16S rRNA sequences. We have shown that clinical isolates could be identified by determining the positions of their sodA int fragments on the phylogenetic tree of the sodA int fragments of the type species. We propose this method for the characterization of strains that cannot be assigned to a species on the basis of their conventional phenotypic reactions.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3