Affiliation:
1. Laboratoire Mixte Pasteur-Necker de Recherche sur les Streptocoques et Streptococcies, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France
Abstract
ABSTRACT
We have used a PCR assay based on the use of degenerate primers in order to characterize an internal fragment (
sodA
int
) representing approximately 85% of the genes encoding the manganese-dependent superoxide dismutase in various streptococcal type strains (
S. acidominimus
,
S. agalactiae
,
S. alactolyticus
,
S. anginosus
,
S. bovis
,
S. constellatus
,
S. canis
,
S. cricetus
,
S. downei
,
S. dysgalactiae
,
S. equi
subsp.
equi
,
S. equi
subsp.
zooepidemicus
,
S. equinus
,
S. gordonii
,
S. iniae
,
S. intermedius
,
S. mitis
,
S. mutans
,
S. oralis
,
S. parasanguis
,
S. pneumoniae
,
S. porcinus
,
S. pyogenes
,
S. salivarius
,
S. sanguis
,
S. sobrinus
,
S. suis
,
S. thermophilus
, and
S. vestibularis
). Phylogenetic analysis of these
sodA
int
fragments yields an evolutionary tree having a topology similar to that of the tree constructed with the 16S rRNA sequences. We have shown that clinical isolates could be identified by determining the positions of their
sodA
int
fragments on the phylogenetic tree of the
sodA
int
fragments of the type species. We propose this method for the characterization of strains that cannot be assigned to a species on the basis of their conventional phenotypic reactions.
Publisher
American Society for Microbiology
Cited by
271 articles.
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