Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111-Reference-Cited by-同舟云学术

Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111

Published:1998-11 Issue:11 Volume:36 Page:3375-3377
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Louie M.¹²,Read S.³,Simor A. E.¹²,Holland J.¹,Louie L.¹,Ziebell K.³,Brunton J.⁴²,Hii J.²

Affiliation:

1. Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre,1

2. University of Toronto,2 Toronto, and

3. Health Canada, Health of Animals Laboratory, Guelph,3 Ontario, Canada

4. The Toronto Hospital,4 and

Abstract

ABSTRACT Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.11.3375-3377.1998

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