Alternate gram staining technique using a fluorescent lectin-Reference-Cited by-同舟云学术

Alternate gram staining technique using a fluorescent lectin

Published:1990-07 Issue:7 Volume:56 Page:2245-2247
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sizemore R K¹,Caldwell J J¹,Kendrick A S¹

Affiliation:

1. Department of Biological Sciences, University of North Carolina, Wilmington 28403-3297.

Abstract

Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.7.2245-2247.1990

Reference11 articles.

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3. Atlas R. M. 1983. Use of microbial diversity measurements to asses environmental stress p. 540-545. In M. J. Klug and C. A. Reddy (ed.) Current perspectives in microbial ecology. American Society for Microbiology Washington D.C.

4. The gram stain;Bartholomew J. W.;Bacteriol. Rev.,1952

5. Gerhardt P. (ed.). 1981. Manual of methods for general bacteriology 4th ed. American Society for Microbiology Washington D.C.

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