Affiliation:
1. Department of Microbiology, Agricultural University, Wageningen, The Netherlands.
Abstract
At 25 degrees C, the optimal temperature for growth of Rhizobium trifolii TA-1, extracellular and capsular polysaccharide (EPS and CPS) were the main carbohydrate products synthesized in mannitol-rich medium (10 g of mannitol and 1 g of glutamic acid per liter). In the same medium at 33 degrees C, EPS and CPS production was inhibited, and up to 3.9 g of cyclic beta-(1,2)-glucan was produced during an incubation period of 20 days with a total biomass of 0.55 g of protein. In a medium containing 50 g of mannitol and 10 g of glutamic acid per liter, high cell densities (3.95 g of protein) were obtained at 25 degrees C. This biomass excreted 10.9 g of cyclic beta-(1,2)-glucan within 10 days. Concomitantly, 4.8 g of EPS were synthesized, while CPS production was strongly suppressed. The excreted cyclic beta-(1,2)-glucans were neutral and had degrees of polymerization ranging from 17 to 25, with a degree of polymerization of 19 as the major glucan cycle.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference25 articles.
1. Studies on cyclic ,-(1,2)-glucan obtained from the periplasmic space of Rhizobium trifolii cells;Abe M.;Plant Soil,1982
2. HPLC of cyclic ,B-(1,2)-glucans (cyclosophoraoses) produced by Rhizobium meliloti and Rhizobium trifolii;Benincasa M.;J. Chromatogr.,1987
3. New method for the quantitative determination of uronic acids;Blumenkrantz M.;Anal. Biochem.,1973
4. The determination of glycerol and dihydroxyacetone;Burton R. M.;Methods Enzymol.,1957
5. A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharides but fails to form 3-(1,2)-glucan;Geremia R. A.;J. Bacteriol.,1987
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