Plate Assay for Simultaneous Detection of Alginate Lyases and Determination of Substrate Specificity-Reference-Cited by-同舟云学术

Plate Assay for Simultaneous Detection of Alginate Lyases and Determination of Substrate Specificity

Published:1990-07 Issue:7 Volume:56 Page:2265-2267
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gacesa Peter¹,Wusteman Frederick S.¹

Affiliation:

1. Department of Biochemistry, University of Wales, P.O. Box 903, Cardiff CF1 1ST, United Kingdom

Abstract

A plate assay to detect the presence of alginate lyases (EC 4.2.2.3) has been developed. The simultaneous use of specific alginate block structures of defined composition allows the substrate specificity of the enzymes to be determined. Clearing zones in the alginate-containing media are visualized with either cetyl pyridinium chloride or ruthenium red.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.7.2265-2267.1990

Reference21 articles.

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4. Properties of alginate lyases from marine bacteria;Doubet R. S.;Appl. Environ. Microbiol.,1984

5. Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis;Dunne W. M.;Appl. Environ. Microbiol.,1985

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