Molecular Detection of Filamentous Fungi in Formalin-Fixed Paraffin-Embedded Specimens in Invasive Fungal Wound Infections Is Feasible with High Specificity-Reference-Cited by-同舟云学术

Molecular Detection of Filamentous Fungi in Formalin-Fixed Paraffin-Embedded Specimens in Invasive Fungal Wound Infections Is Feasible with High Specificity

Published:2019-12-23 Issue:1 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ganesan Anuradha¹²³,Wells Justin³,Shaikh Faraz¹²,Peterson Philip³,Bradley William¹²⁴,Carson M. Leigh¹²,Petfield Joseph L.⁵,Klassen-Fischer Mary⁶,Akers Kevin S.⁷,Downing Kevin⁵,Bialek Ralf⁸,Tribble David R.¹,Wickes Brian L.⁹

Affiliation:

1. Infectious Disease Clinical Research Program, Preventive Medicine and Biostatistics Department, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

2. Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA

3. Walter Reed National Military Medical Center, Bethesda, Maryland, USA

4. Brooke Army Medical Center, JBSA Fort Sam Houston, Texas, USA

5. Landstuhl Regional Medical Center, Landstuhl, Germany

6. Joint Pathology Center, Silver Spring, Maryland, USA

7. U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, Texas, USA

8. LADR GmbH Medizinisches Versorgungszentrum Dr. Kramer und Kollegen, Geesthacht, Germany

9. University of Texas Health Sciences Center at San Antonio, San Antonio, Texas, USA

Abstract

Trauma-related invasive fungal wound infections (IFIs) are associated with significant morbidity and mortality. Early identification and treatment are critical. Traditional identification methods (e.g., fungal cultures and histopathology) can be delayed and insensitive. We assessed a PCR-based sequencing assay for rapid identification of filamentous fungi in formalin-fixed paraffin-embedded (FFPE) specimens obtained from combat casualties injured in Afghanistan.

Funder

Department of Navy under Wounded, Ill, and Injured Program

Defense Medical Research and Development Program

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01259-19

Reference38 articles.

1. Invasive Mold Infections Following Combat-related Injuries