Fusobacterium nucleatum Subspecies Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Abstract

We explored the use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for identification ofFusobacterium nucleatumsubspecies. MALDI-TOF MS spectra of fiveF. nucleatumsubspecies (animalis,fusiforme,nucleatum,polymorphum, andvincentii) were analyzed and divided into four distinct clusters, including subsp.animalis,nucleatum,polymorphum, andfusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34F. nucleatumclinical isolates to the subspecies level.