Functional expression of plastid allophycocyanin genes in a cyanobacterium

Author:

de Lorimier R,Guglielmi G,Bryant D A,Stevens S E

Abstract

In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa allophycocyanin subunits. Thus, these plastid genes are expressed in the cyanobacterium as polypeptides which become linked to a chromophore and are incorporated into the light-harvesting apparatus.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

1. Bryant D. A. R. de Lorimier G. Guglielmi V. L. Stirewalt J. M. Dubbs B. Ilhman G. Gasparich J. S. Buzby A. Cantrell R. C. Murphy J. Gingrich R. D. Porter and S. E. Stevens Jr. 1986. The cyanobacterial photosynthetic apparatus: a molecular genetics analysis p. 39-46. In D. C. Youvan and F. Daldal (ed.) Microbial energy transduction: genetics structure and function. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

2. Molecular cloning and nucleotide sequence of the a and 3 subunits of allophycocyanin from the cyanelle genome of Cyanophora paradoxa;Bryant D. A.;Proc. Natl. Acad. Sci. USA,1985

3. Expression of phycobiliprotein genes in Escherichia coli;Bryant D. A.;FEMS Microbiol. Lett.,1985

4. The structure of cyanobacterial phycobilisomes: a model;Bryant D. A.;Arch. Microbiol.,1979

5. Plasmid transformation in Agmenellum quadruplicatum PR-6: construction of biphasic plasmids and characterization of their transformation properties;Buzby J. S.;J. Bacteriol.,1983

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