Further mapping of several membrane lipid biosynthetic genes (fabC, fabB, gpsA, plsB) of Escherichia coli

Abstract

New genetic mapping data on several loci involved in membrane lipid synthesis are reported. We demonstrated that the lesion designated fabC by Broekman and Hoeckstra (Mol. Gen. Genet. 124:65-67, 1975) is a fabB mutation. In the course of this work, the orientation of the pdxB and fabB loci was determined. The order of the loci is fabB pdxB purF. We also report cotransduction between the gpsA and cysE loci and show that the order of these markers is mtl cysE gpsA. Cotransduction between the plsB and kdgK loci was also sought. Despite extensive experiments, we were unable to detect cotransduction between these loci. In addition, we were unable to detect cotransduction among several markers in region 46 to 48 min of the map.