Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays-Reference-Cited by-同舟云学术

Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Published:2005-05 Issue:5 Volume:12 Page:683-684
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Velázquez Blanca¹²,Massaldi Hugo¹²,Battistoni Julio¹²,Chabalgoity José A.¹²

Affiliation:

1. Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay

2. Laboratory of Immunotechnology, Department of Immunology, School of Chemistry-School of Science, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay

Abstract

ABSTRACT Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.12.5.683-684.2005

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