Affiliation:
1. Department of Microbiology, The University of Iowa, Iowa City, Iowa 52240
Abstract
The fatty acid content of
Thiobacillus novellus
was determined under various cultural conditions. Four fatty acids, C
16:0
, C
18:0
, C
18:1
, and a C
19
cyclopropane acid (C
19:cyc
), generally accounted for 90 to 99% of the total acids. Phosphate concentration, temperature, culture agitation, and the presence of branch-chain precursors had no significant effect on cellular fatty acids. Autotrophically grown cells contained more saturated C
16
and C
18
acids than did heterotrophic ones, and the sum of the percentages of the C
18:1
and the C
19:cyc
acids was consistently higher in the heterotrophs. When the inorganic medium was supplemented with biotin, autotrophic cells produced more C
19:cyc
and much less C
18:1
than did autotrophs in unsupplemented medium. Heterotrophic cells grown with biotin also showed a marked reduction of the unsaturated acid and an increase in the cyclopropane acid, except when glutamatecitrate medium was employed, in which case the opposite effect was noted. Two different biotin-supplemented media yielded cells with 75 to 77% of the total fatty acids being the C
19
cyclopropane acid, one of the highest reported values for this class of acid.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
17 articles.
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