Field Evaluation of a Novel Colorimetric Method—Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay—To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand-Reference-Cited by-同舟云学术

Field Evaluation of a Novel Colorimetric Method—Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay—To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

Published:2004-04 Issue:4 Volume:48 Page:1426-1429
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Brockman Alan¹²³,Singlam Sittaporn¹²,Phiaphun Lucy¹,Looareesuwan Sornchai²,White Nicholas J.²⁴,Nosten François¹²⁴

Affiliation:

1. Shoklo Malaria Research Unit, Mae Sot, Tak 63110

2. Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand

3. Australian Centre for International Tropical Health and Nutrition, University of Queensland, Brisbane, Australia

4. Centre for Tropical Diseases, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom

Abstract

ABSTRACT A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.4.1426-1429.2004

Reference7 articles.

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