Effect of Amoxicillin-Clavulanic Acid on Human Fecal Flora in a Gnotobiotic Mouse Model Assessed with Fluorescence Hybridization Using Group-Specific 16S rRNA Probes in Combination with Flow Cytometry-Reference-Cited by-同舟云学术

Effect of Amoxicillin-Clavulanic Acid on Human Fecal Flora in a Gnotobiotic Mouse Model Assessed with Fluorescence Hybridization Using Group-Specific 16S rRNA Probes in Combination with Flow Cytometry

Published:2004-04 Issue:4 Volume:48 Page:1365-1368
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Barc Marie Claude¹,Bourlioux François¹,Rigottier-Gois Lionel²,Charrin-Sarnel Céline¹,Janoir Claire¹,Boureau Hélène¹,Doré Joël²,Collignon Anne¹

Affiliation:

1. Unité associée INRA “Ecosystème Microbien Digestif et Santé,” Faculté de Pharmacie, Université Paris-Sud, 92296 ChÂtenay-Malabry Cedex

2. Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78852 Jouy en Josas Cedex, France

Abstract

ABSTRACT Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.4.1365-1368.2004

Reference27 articles.

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