Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d -Cycloserine in a Silkworm Infection Model-Reference-Cited by-同舟云学术

Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d -Cycloserine in a Silkworm Infection Model

Published:2009-09 Issue:9 Volume:53 Page:4025-4027
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kurokawa Kenji¹²,Hamamoto Hiroshi¹,Matsuo Miki¹,Nishida Satoshi¹,Yamane Noriko³,Lee Bok Luel²,Murakami Kazuhisa³,Maki Hideki³,Sekimizu Kazuhisa¹

Affiliation:

1. Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

2. National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Jangjeon-dong, Geumjeong-gu, Busan 609-735, South Korea

3. Discovery Research Laboratories, Shionogi and Co., Ltd., 3-1-1 Futaba-Cho, Toyonaka, Osaka 561-0825, Japan

Abstract

ABSTRACT The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED 50 ) of d -cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED 50 of other antibiotics were negligible, suggesting that these alterations are d -cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00200-09

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