Affiliation:
1. Research Station, Agriculture Canada, Sainte-Foy, Quebec, Canada GIV 2J3
Abstract
Whey, a by-product of the cheese industry, can sustain the growth of fast-growing rhizobia. To avoid any latency of growth, rhizobial inoculum must be prepared under inducing conditions. In unsupplemented whey, the number of cells of
Rhizobium meliloti
Balsac reached 5 × 10
9
CFU/ml in 48 h of incubation. This is comparable to the yield obtained with yeast-mannitol broth, the standard medium for the growth of rhizobia. In raw whey supplemented with yeast extract (1.0 g/liter) and phosphate (0.5 g/liter), the number of cells reached 10
10
CFU/ml in 48 h of incubation. This is a twofold increase compared with the population normally obtained in industrial production. Whey represents a relatively inexpensive and efficient substrate medium for the large-scale production of fast-growing rhizobia.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference21 articles.
1. Glycerol metabolism in Rhizobium;Arias A.;Can. J. Microbiol.,1976
2. Rhizobium biomass production in batch and continuous culture with a malt-sprouts medium;Boiardi J. L.;MIRCEN J. Appl. Microbiol. Biotechnol.,1985
3. Effets des souches de Rhizobium meliloti et des coupes successives de la Iuzerne (Medicago sativa) sur la fixation symbiotique d'azote;Bordeleau L. M.;Can. J. Plant Sci.,1977
4. Bremner J. M. 1965. Organic forms of nitrogen p. 1236-1255. In C. A. Black D. D. Evans J. L. White L. E. Ensminger and F. E. Clark (ed.) Methods of soil analysis part 2. Chemical and microbiological properties. American Society of Agronomy Madison Wis.
5. Burton J. C. 1979. Rhizobium species p. 29-58. In H. J. Peppler (ed.) Microbial technology. Academic Press Inc. New York.
Cited by
20 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献