Comparative Secretome Analyses of Three Bacillus anthracis Strains with Variant Plasmid Contents

Author:

Lamonica Janine M.1,Wagner MaryAnn1,Eschenbrenner Michel1,Williams Leanne E.1,Miller Tabbi L.1,Patra Guy1,DelVecchio Vito G.1

Affiliation:

1. Institute of Molecular Biology and Medicine, The University of Scranton, 800 Linden Street, Scranton, Pennsylvania 18510

Abstract

ABSTRACT Bacillus anthracis , the causative agent of anthrax, secretes numerous proteins into the extracellular environment during infection. A comparative proteomic approach was employed to elucidate the differences among the extracellular proteomes (secretomes) of three isogenic strains of B. anthracis that differed solely in their plasmid contents. The strains utilized were the wild-type virulent B. anthracis RA3 (pXO1 + pXO2 + ) and its two nonpathogenic derivative strains: the toxigenic, nonencapsulated RA3R (pXO1 + pXO2 ) and the totally cured, nontoxigenic, nonencapsulated RA3:00 (pXO1 pXO2 ). Comparative proteomics using two-dimensional gel electrophoresis followed by computer-assisted gel image analysis was performed to reveal unique, up-regulated, or down-regulated secretome proteins among the strains. In total, 57 protein spots, representing 26 different proteins encoded on the chromosome or pXO1, were identified by peptide mass fingerprinting. S-layer-derived proteins, such as Sap and EA1, were most frequently observed. Many sporulation-associated enzymes were found to be overexpressed in strains containing pXO1 + . This study also provides evidence that pXO2 is necessary for the maximal expression of the pXO1-encoded toxins lethal factor (LF), edema factor (EF), and protective antigen (PA). Several newly identified putative virulence factors were observed; these include enolase, a high-affinity zinc uptake transporter, the peroxide stress-related alkyl hydroperoxide reductase, isocitrate lyase, and the cell surface protein A.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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