Affiliation:
1. Danisco Cultures Division, Waukesha, Wisconsin
Abstract
ABSTRACT
A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect
Propionibacterium acidipropionici
P169, with a threshold of 10
4
CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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