Affiliation:
1. Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, Universidad de Sevilla-CSIC, E-41092 Sevilla, Spain
Abstract
ABSTRACT
A gene cluster composed of nine open reading frames (ORFs) involved in Ni
2+
, Co
2+
, and Zn
2+
sensing and tolerance in the cyanobacterium
Synechocystis
sp. strain PCC 6803 has been identified. The cluster includes an Ni
2+
response operon and a Co
2+
response system, as well as a Zn
2+
response system previously described. Expression of the Ni
2+
response operon (
nrs
) was induced in the presence of Ni
2+
and Co
2+
. Reduced Ni
2+
tolerance was observed following disruption of two ORFs of the operon (
nrsA
and
nrsD
). We also show that the
nrsD
gene encodes a putative Ni
2+
permease whose carboxy-terminal region is a metal binding domain. The Co
2+
response system is composed of two divergently transcribed genes,
corR
and
corT
, mutants of which showed decreased Co
2+
tolerance. Additionally,
corR
mutants showed an absence of Co
2+
-dependent induction of
corT
, indicating that CorR is a transcriptional activator of
corT
. To our knowledge, CorR is the first Co
2+
-sensing transcription factor described. Our data suggest that this region of the
Synechocystis
sp. strain PCC 6803 genome is involved in sensing and homeostasis of Ni
2+
, Co
2+
, and Zn
2+
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
86 articles.
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