Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field

Author:

Heitkamp M A1,Cerniglia C E1

Affiliation:

1. National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.

Abstract

Microbiological analyses of sediments chronically exposed to petrogenic hydrocarbons resulted in the isolation of a gram-positive, rod-shaped bacterium which mineralized naphthalene (59.5% of the original amount), phenanthrene (50.9%), fluoranthene (89.7%), pyrene (63.0%), 1-nitropyrene (12.3%), 3-methylcholanthrene (1.6%), and 6-nitrochrysene (2.0%) to carbon dioxide when grown for 2 weeks in pure culture with organic nutrients. The bacterium tolerated salt concentrations up to 4% and grew well at 24 to 30 degrees C. The use of this bacterium may be an attractive alternative to existing physicochemical methods for the remediation of polycyclic aromatic hydrocarbons in the environment.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference12 articles.

1. Microbial metabolism of polycyclic aromatic hydrocarbons;Cerniglia C. E.;Adv. Appl. Microbiol.,1984

2. Microbial degradation of hydrocarbons;Gibson D. T.;Toxicol. Environ. Chem.,1982

3. Gibson D. T. and V. Subramanian. 1984. Microbial degradation of aromatic hydrocarbons p. 181-252. In D. T. Gibson (ed.) Microbial degradation of organic compounds. Marcel Dekker Inc. New York.

4. Effects of chemical structure and exposure on the microbial degradation of polycyclic aromatic hydrocarbons in freshwater and estuarine ecosystems;Heitkamp M. A.;Environ. Toxicol. Chem.,1987

5. Naphthalene biodegradation in environmental microcosms: estimates of degradation rates and characterization of metabolites;Heitkamp M. A.;Appl. Environ. Microbiol.,1987

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